New PDF release: Cyanoprokaryota: Teil 1 / Part 1: Chroococcales

By Jiří Komárek

ISBN-10: 3437304038

ISBN-13: 9783437304033

- Band 2/1 der Süßwasserflora von Mitteleuropa hat sich weltweit als bisher unübertroffenes Bestimmungswerk erwiesen - Praktisch unverzichtbar für Phykologen, Ökologen, Hydrobiologen und Geologen - Der Allgemeine Teil von 2/1 ist unverändert aktuell - Der

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Additional resources for Cyanoprokaryota: Teil 1 / Part 1: Chroococcales

Example text

Insertion of foreign genes into plant viral genomes can be achieved using the following methods: 1. Gene replacement, in which nonessential viral genes such as the coat protein gene are replaced by the gene of interest. 2. Gene insertion, in which the gene of interest is placed under the control of an additional strong subgenomic promoter. 3. Gene fusion, in which the gene of interest is translationally fused with a viral gene (8,12). Among plant RNA viruses, PVX is widely used for expressing virulence and avirulence genes from viruses, bacteria, fungi, and oomycetes.

Coli DH5α cells. 6. In Silico Screening 1. org. 2. html for free. 3. Any web browser to access online databases. 3. Methods We describe here how to use the minitransposon pDSG50 that carries the AvRpt281-255 reporter in P. syringae on A. thaliana. This construct was used in the effector screen described in Guttman et al. (10). We describe in Note 5 how to substitute the AvRpt281-255 reporter with other reporters to apply the in vivo screen to other pathogens on other plants. 1. Mating the Minitransposon Into P.

Make sure that the reporter is sufficient to elicit an HR inside the plant cell. Create defined effector::reporter fusions to make sure fusions to your reporter elicit a strong HR on your host plant. Also perform setup experiments to identify the optimal infection dose, plant cultivar/ecotype, age of plants, and growth conditions to reach the highest possible sensitivity in your screen. It is also useful to add an epitope tag to your reporter if you do not have an antibody against it (see Note 9).

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Cyanoprokaryota: Teil 1 / Part 1: Chroococcales by Jiří Komárek


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