Download e-book for iPad: Culture of human stem cells by R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach

By R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach

ISBN-10: 0470052465

ISBN-13: 9780470052464

This book collects the best and state-of-the-art equipment and protocols for deriving and culturing human embryonic and grownup stem cells—in one convenient source.

This groundbreaking book follows the culture of earlier books within the tradition of specialised Cells sequence —each tools and protocols bankruptcy is laid out precisely just like the subsequent, with stepwise protocols, preceded via particular requisites for that protocol, and a concise dialogue of equipment illustrated through info. The editors describe a constrained variety of consultant options throughout a large spectrum of stem cells from embryonic, child, and grownup tissue, yielding an all-encompassing and flexible consultant to the sector of stem phone biology and tradition.

The ebook features a complete checklist of providers for all gear utilized in the protocols provided, with web pages to be had in an appendix. also, there's a bankruptcy on quality controls, and different chapters protecting criminal and moral concerns, cryopreservation, and feeder layer culture. This textual content is a one-stop source for all researchers, medical scientists, lecturers, and scholars keen on this significant region of analysis.

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5) and easily grown in culture, providing a reliable and consistent source of feeders that can be maintained for extended periods in liquid nitrogen. MEFs are typically ready for use within 2–5 days after thawing. 1 has been adapted from Nagy et al. [2003]. 1. 5-day pregnant mouse. Wash the mouse abdomen thoroughly with 70% alcohol. Cut through the skin and tissue to expose the uterus. Remove the uterine horns and place in a 10-cm plastic Petri dish containing PBSA. Remove the embryos from the embryonic sac with a sterile scalpel and discard all other tissue including the placenta and membranes.

2. Characterization of hES Cells by Polymerase Chain Reaction Analysis . . . 12. RT-PCR Synthesis of cDNA in hES Cells . . . . . . . . . . . . . . . 13. Analysis of Gene Expression in hES Cells by PCR . . . . . . . . . 3. Karyotyping hES Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8. Differentiation Studies Using hES Cells . . . . . . . . . . . . . . . . .

1. Derivation and Cultivation of Human ES Cells . . . . . . . . . . . . . . . . . 2. Propagation of Human ES Cells. . . . . . . . . . . . . . . . . . . . . . . . . 3. Differentiation of Human Embryonic Stem Cells . . . . . . . . . . . . . . . . 2. Cell Culture Medium Components. . . . . . . . . . . . . . . . . . . . . . . . . . . 1. Human Embryonic Stem (hES) Cell Medium . . . . . . . . . . . . . .

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Culture of human stem cells by R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach


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