By Harry J. Gilbert (Eds.)
Cellulase refers to a category of enzymes produced mainly through fungi, micro organism, and protozoans that catalyze cellulolysis. This quantity of Methods in Enzymology comprehensively covers this subject. With a world board of authors, this quantity covers topics such as ''The DNSA lowering assay for measuring cellulases,'' ''Measuring processivity'' and ''In situ cellulose detection with carbohydrate-binding modules.''
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Typical standard curves for the hydrolysis of Cellazyme C tablets (containing dyed and crosslinked HP-cellulose) and Beta-Glucazyme tablets (containing dyed and crosslinked barley b-glucan) are shown in Figs. e. 6 Standard curve for the action of Trichoderma longibrachiatum EG on Cellazyme C tablets (Lot 80201). 5) was preincubated at 40 C for 5 min. A Cellazyme C tablet was then added (without stirring) and the reaction allowed to proceed for exactly 10 min. The reaction was terminated by the addition of 10 ml of 2% Trizma Base (pH $ 9) and the tube contents mixed vigorously on a vortex stirrer.
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Cellulases by Harry J. Gilbert (Eds.)