By Mark D. Adams, Chris Fields, J. Craig Venter
A well timed booklet for DNA researchers, automatic DNA Sequencing and research stories and assesses the cutting-edge of computerized DNA series analysis-from the development of clone libraries to the developmentof laboratory and group databases. It provides the methodologies and techniques of computerized DNA series research in a fashion that enables them to be in comparison and contrasted. by means of taking a huge view of the technique of computerized series research, the current quantity bridges the space among the protocols provided with device and response kits and the finalized info awarded in th. Read more...
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Extra info for Automated DNA Sequencing and Analysis
1990a; S. Surzyski , personal communication ). Th ese meth ods can be grouped into th ree categories: direct ed clonin g (Sa nger et al. , 1980; Banki er et al. , 1987; Phadnis et al. , 1990a; Strat hmann et al. , 1991; Fitzgera ld et al. , 1992; S. Surzyski, persona l comm unicatio n) . In the directed cloning approach, fragme nts of the DNA of intere st are digested with a se ries of restriction endonucleases and cloned directly into M13, pUC or oth er vectors with the appropriate unique cloning sites and selection methods.
The complement arity of the two meth ods can be furth er broadened by extending the list of probes to appr oximat ely 3000, mainly 7-mers (Drm anac , 1992) . By the described SBH technology, 1 million clones (covering 109 bp) could be analyzed with such a number of prob es within about two years in one laboratory (Drmanac et al. , 1992a). Th e basic idea is to determine the complete and correct sequences by combining low-redundancy dat a from the two methods . With 3000 6-mers and 7-mers, every base is going to be read on averag e four times, but positively scored prob es can not be correctly ordered .
Chern. 64, 967-9 72. c. A . A . (1992b) An al. Chern. 64, 2149-2154 . F. & Hood , L. (1991) Science 254, 59-6 7. , D'Cunh a, J. B. M . (1990) Nucleic A cids Res . 18,4417-4421. A . c. (1992) Nature (l-on don) 359, 167-169. L. & Gra bherr, R. (1992) Nucleic Acids Res. 20, 1345-13 48. L. D . (1992) Proceedings of CLEOIQE LS . p. 94. C. L. J. W. A . & Baumeister, K. (1987) Science 238, 336--341. c. N. A . (1991) BioTechniqu es 10, 616--625. C. A. , Yue , S. A . P . A. N. (1992) Methods Enzy mol. 217, 414-431.
Automated DNA Sequencing and Analysis by Mark D. Adams, Chris Fields, J. Craig Venter