Annual Update in Intensive Care and Emergency Medicine 2012 - download pdf or read online

By W. F. Carson IV, S. L. Kunkel (auth.), Prof. Jean-Louis Vincent (eds.)

ISBN-10: 3642257151

ISBN-13: 9783642257155

ISBN-10: 364225716X

ISBN-13: 9783642257162

The Yearbook compiles the newest advancements in experimental and medical study and perform in a single complete reference booklet. The chapters are written by way of good well-known specialists within the box of extensive care and emergency medication. it's addressed to every person serious about inner medication, anesthesia, surgical procedure, pediatrics, extensive care and emergency medicine.

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Flow cytometric analysis can be performed using various techniques during the subsequent 24 hours – if the tubes are kept at 4 °C – without causing variation in the results. The procedure consists of an initial double labeling of the cells with two antibodies: Anti-CD14 – a specific marker for monocytes – and anti-HLA-DR, usually coupled with phycoerythrin. Analysis by flow cytometry allows for identification of monocytes based on CD14 expression. mHLA-DR expression is then evaluated in these cells using the fluorescence of phycoerythrin (Fig.

In addition, identification of epigenetic marks associated with disease severity may serve as useful biomarkers for the diagnosis of patients at high risk of succumbing to sepsis-induced mortality, or for developing longterm immunosuppression following recovery. Further research into the role of epigenetics in sepsis will hopefully provide new clues into the molecular mechanisms governing the disease, as well as providing novel targets for the development of pharmacological treatments. Acknowledgement: This work was supported in part by NIH grant HL31237 References 1.

J Immunol 175: 461 – 468 23. El Gazzar M, Yoza BK, Chen X, et al (2008) G9a and HP1 couple histone and DNA methylation to TNFalpha transcription silencing during endotoxin tolerance. J Biol Chem 283: 32198 – 32208 24. Trinchieri G (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat Rev Immunol 3: 133 – 146 25. Kasten KR, Tschop J, Adediran SG, Hildeman DA, Caldwell CC (2010) T cells are potent early mediators of the host response to sepsis. Shock 34: 327 – 336 26.

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Annual Update in Intensive Care and Emergency Medicine 2012 by W. F. Carson IV, S. L. Kunkel (auth.), Prof. Jean-Louis Vincent (eds.)

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