Download e-book for iPad: ABC Transporters: Biochemical, Cellular, and Molecular by John N. Abelson, Melvin I. Simon, Suresh V. Ambudkar,

By John N. Abelson, Melvin I. Simon, Suresh V. Ambudkar, Michael M. Gottesman

ISBN-10: 0076687929

ISBN-13: 9780076687923

ISBN-10: 0121821935

ISBN-13: 9780121821937

The seriously acclaimed laboratory regular for greater than 40 years, Methods in Enzymology is without doubt one of the such a lot hugely revered courses within the box of biochemistry. considering that 1955, every one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. greater than 285 volumes were released (all of them nonetheless in print) and lots more and plenty of the fabric is proper even today-truly a necessary ebook for researchersin all fields of lifestyles sciences.

Key Features
* Prokaryotic ABC Transporters
* Eukaryotic ABC Transporters
* Nonmammalian ABC shipping Systems
* Mammalian P-Glycoproteins
* Multidrug Resistance linked Protein
* Cystic Fibrosis Transmembrane Conductance Regulator
* Sulfonylurea Receptor
* Intracellular ABC Transporters

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Additional info for ABC Transporters: Biochemical, Cellular, and Molecular Aspects

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Natl. Acad. Sci. USA 84, 8525 (1987). ~ E. Dassa and S. Muir, Mol. Microbiol. 7, 29 (1993). t7 E. Gilson, H. Nikaido, and M. Hofnung, Nucl. Acids Res. 10, 7449 (1982). ~sS. Morbach, S. Tebbe, and E. Schneider, J. Biol. Chem. 268, 18617 (1993). t9 P. Bavoil, M. Hofnung, and H. Nikaido, J. Biol. Chem. 255, 8366 (1980). 2oH. A. Shuman and T. J. Silhavy, J. Biol. Chem. 256, 560 (1981). 32 PROKARYOTICABC TRANSPORTERS [31 m e m b r a n e . 21 Purification of the MalFGK2 complex has been described, 22,23 and the stoichiometry and enzymatic properties of the complex have been investigated.

Addition of liganded MBP stimulated A T P hydrolysis with some mutants, but caused inhibition in a few mutants. A model of the mechanism of transport suggested by these data is described in the following section. M e c h a n i s m of T r a n s p o r t t h r o u g h ABC T r a n s p o r t e r s In the proteoliposomes containing the wild-type transporter, A T P hydrolysis does not occur unless liganded MBPs are present on the other side of the membranes. The simplest interpretation is that liganded MBPs bind to the periplasmic surface of the transporter, and this association creates a wave of conformational changes that travels the thickness of the membrane through the integral membrane proteins MalF and MalG, so that the A T P hydrolysis can now be catalyzed by the ATP-binding subunit MalK, located on the other, cytoplasmic, side of the membrane (see Fig.

37 E. Schneider and C. Walter, Mol. Microbiol. 5, 1375 (1991). 3~ S. C. Hyde, P. Emsley, M. J. Hartshorn, M. M. Mimmack, U. Gileadi, S. R. Pearce, M. P. Gallagher, D. R. Gill, R. E. Hubbard, and C. Higgins, Nature 345, 362 (1990). 39 C. S. Mimura, S. R. Holbrook, and G. -L. A m e s , Proc. NatL Acad. Sci. USA 88, 84 (1991). 10 PROKARYOTIC ABC TRANSPORTERS [11 The N-terminal part containing the Walker motif A and the C-terminal part containing the Walker motif B (Fig. 1) are predicted to fold together in a way similar to the model protein and to produce the ATP-binding pocket, but the large, middle part of the protein containing about 90 amino acid residues was excluded entirely from this pocket structure in at least one model.

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ABC Transporters: Biochemical, Cellular, and Molecular Aspects by John N. Abelson, Melvin I. Simon, Suresh V. Ambudkar, Michael M. Gottesman


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